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Pyrosequencing wasn’t developed early enough to be employed by the Human Genome Project or Celera, but it was still the first method other than Sanger sequencing to hit the commercial market, marking the start of “next generation” sequencing methods (NGS). Pyrosequencing worked in real-time, though it struggled to accurately capture regions with several of the same nucleotides in a row. This was because the amount of light didn’t always scale cleanly when pyrophosphate was produced through successive reactions.
,更多细节参见下载安装 谷歌浏览器 开启极速安全的 上网之旅。
Note that I left out some implementation details to focus on the main idea. For a practical implementation you would,这一点在爱思助手下载最新版本中也有详细论述
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